Second harmonic generation microscopy gets better contrast
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Zebrafish is a well establish model for studying development of the vascular system. Multiphoton imaging is a great way to capture the intricate vasculature patterns in zebrafish brain at great depth. Additionally, through the second-harmonic generation, structural information of the surrounding tissues can be captured without the need of additional labelling. In this video, you can see the zebrafish brain & eye vasculature (green) and second-harmonic generation (grey) in sagittal orientation. I
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